By Su-Sen Chang; Dong-Hyun Kang

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Extra resources for Alicyclobacillus spp. in the Fruit Juice Industry: History, Characteristics, and Current Isolation/Detection Procedures

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The absorption spectrum of the clarified liquid is measured between 200 and 600 nm. There are six main maxima of interest which can be used to characterise the spectrum quantitatively. Petrus also indicates that a practised qualitative assessment of the whole spectrum is valuable. Fluorescence excitation and emission spectra have been used by the same workers in conjunction with the ultraviolet and visible absorption AUTHENTICATION OF ORANGE JUICE 17 measurements. The tluorescence spectra are mainly a check on the indications from the more easily measured absorption spectra.

The heavy isotope of hydrogen is deuterium, the abundance of which is low (about 150 per million hydrogen atoms), but still measurable. 4, isotopic fractionation effects influence the deuterium content of natural products, providing information on the botanical and even geographical origin of the plant in which the molecule has been synthesised. Deuterium isotope ratios, (D/H) are generally determined by IRMS, or isotope ratio mass spectrometry methods, expressed as a deviation relative to SMOW (standard mean ocean water) as the international standard.

Despite the above, most work in Europe still uses the dedicated ninhydrinbased analyser, and gas chromatography also has some supporters. Whatever technique is chosen will involve substantial capital expenditure and require skilled staff. However, the comprehensive results obtainable are valuable in detection of the most sophisticated falsifications. The analysis is not wholly straightforward. Current attempts to harmonise methods of orange juice analysis within the EEC have shown that while laboratories are usually self-consistent, large discrepancies (coefficients of variation of25% and more) can occur between them.

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Alicyclobacillus spp. in the Fruit Juice Industry: History, Characteristics, and Current Isolation/Detection Procedures by Su-Sen Chang; Dong-Hyun Kang


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